Tools
Tools
Handley, Rebecca A, Mulholland, Francis, Reuter, Mark, Ramachandran, Vinoy K, Musk, Heather, Clissold, Leah, Le Brun, Nick E 
ORCID: https://orcid.org/0000-0001-9780-4061 and van Vliet, Arnoud H M
(2015)
PerR controls oxidative stress defence and aerotolerance but not motility-associated phenotypes of Campylobacter jejuni.
Microbiology, 161.
pp. 1524-1536.
ISSN 1350-0872
Preview |
PDF (Handley_2015_Microbiology_accepted version)
- Accepted Version
Download (2MB) | Preview |
Abstract
The foodborne bacterial pathogen Campylobacter jejuni is an obligate microaerophile, which is exposed to atmospheric oxygen during transmission through the food chain. Survival under aerobic conditions requires the concerted control of oxidative stress systems, which in C. jejuni are intimately connected with iron metabolism via the PerR and Fur regulatory proteins. Here we have characterised the roles of C. jejuni PerR in oxidative stress- and motility phenotypes, and its regulon at the level of transcription, protein expression and promoter interactions. Insertional inactivation of perR in the C. jejuni reference strains NCTC 11168, 81-176 and 81116 did not result in any growth deficiencies, but strongly increased survival in atmospheric oxygen conditions, and allowed growth around filter discs infused with up to 30% H2O2 (8.8 M). Expression of catalase, alkyl hydroperoxide reductase, thioredoxin reductase and the Rrc desulforubrerythrin were increased in the perR mutant, and this was mediated at the transcriptional level as shown by electrophoretic mobility shift assays of the katA, ahpC and trxB promoters using purified PerR. Differential RNA-seq analysis of a fur perR mutant allowed the identification of eight previously unknown transcription start sites of genes controlled by either Fur and/or PerR. Finally, inactivation of perR in C. jejuni did not result in reduced motility, and did not reduce killing of Galleria melonella wax moth larvae. In conclusion, PerR plays an important role in controlling oxidative stress resistance and aerobic survival of C. jejuni, but this role does not extend into control of motility and associated phenotypes.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes |
| Depositing User: | Pure Connector |
| Date Deposited: | 01 Dec 2015 07:22 |
| Last Modified: | 18 Oct 2024 23:41 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/55519 |
| DOI: | 10.1099/mic.0.000109 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |