Standards for plant synthetic biology: a common syntax for exchange of DNA parts

Patron, Nicola J., Orzaez, Diego, Marillonnet, Sylvestre, Warzecha, Heribert, Matthewman, Colette, Youles, Mark, Raitskin, Oleg, Leveau, Aymeric, Farré, Gemma, Rogers, Christian, Smith, Alison, Hibberd, Julian, Webb, Alex A. R., Locke, James, Schornack, Sebastian, Ajioka, Jim, Baulcombe, David C., Zipfel, Cyril, Kamoun, Sophien ORCID: https://orcid.org/0000-0002-0290-0315, Jones, Jonathan D. G., Kuhn, Hannah, Robatzek, Silke, Van Esse, H. Peter, Sanders, Dale, Oldroyd, Giles, Martin, Cathie, Field, Rob, O'Connor, Sarah, Fox, Samantha, Wulff, Brande, Miller, Ben ORCID: https://orcid.org/0000-0003-0882-033X, Breakspear, Andy, Radhakrishnan, Guru, Delaux, Pierre-Marc, Loqué, Dominique, Granell, Antonio, Tissier, Alain, Shih, Patrick, Brutnell, Thomas P., Quick, W. Paul, Rischer, Heiko, Fraser, Paul D., Aharoni, Asaph, Raines, Christine, South, Paul F., Ané, Jean-Michel, Hamberger, Björn R., Langdale, Jane, Stougaard, Jens, Bouwmeester, Harro, Udvardi, Michael, Murray, James A. H., Ntoukakis, Vardis, Schäfer, Patrick, Denby, Katherine, Edwards, Keith J., Osbourn, Anne and Haseloff, Jim (2015) Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist, 208 (1). pp. 13-19. ISSN 0028-646X

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Abstract

Inventors in the field of mechanical and electronic engineering can access multitudes of components and, thanks to standardization, parts from different manufacturers can be used in combination with each other. The introduction of BioBrick standards for the assembly of characterized DNA sequences was a landmark in microbial engineering, shaping the field of synthetic biology. Here, we describe a standard for Type IIS restriction endonuclease-mediated assembly, defining a common syntax of 12 fusion sites to enable the facile assembly of eukaryotic transcriptional units. This standard has been developed and agreed by representatives and leaders of the international plant science and synthetic biology communities, including inventors, developers and adopters of Type IIS cloning methods. Our vision is of an extensive catalogue of standardized, characterized DNA parts that will accelerate plant bioengineering.

Item Type:	Article
Additional Information:	© 2015 The Authors. New Phytologist © 2015 New Phytologist Trust.
Faculty \ School:	Faculty of Science Faculty of Science > School of Biological Sciences Faculty of Science > School of Computing Sciences
Depositing User:	Pure Connector
Date Deposited:	30 Oct 2015 14:00
Last Modified:	21 Oct 2022 01:06
URI:	https://ueaeprints.uea.ac.uk/id/eprint/54962
DOI:	10.1111/nph.13532

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