Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

Zhao, X., Moates, G. K., Elliston, A., Wilson, D. R., Coleman, M. and Waldron, K. W. (2015) Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre. Bioresource Technology, 194. 263–269. ISSN 1873-2976

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Abstract

This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using CellicÒ CTec 2 (20 U or 0.87 FPU gﰂ1 substrate) cellulase plus b-glucosidase (2 U gﰂ1 substrate) and a yeast inoculum of 10% (v/v or 8.0 ﰀ 107 cells mLﰂ1). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concen- tration of 20% (w/v) by metabolising fermentation inhibitors.

Item Type:	Article
Additional Information:	Copyright 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
Uncontrolled Keywords:	duckweed,bioethanol production,steam explosion,cellulase,simultaneous saccharification ,fermentation
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences Faculty of Science > Research Groups > Biosciences Teaching and Education Research
Depositing User:	Pure Connector
Date Deposited:	25 Aug 2015 09:20
Last Modified:	28 Jun 2025 00:21
URI:	https://ueaeprints.uea.ac.uk/id/eprint/54118
DOI:	10.1016/j.biortech.2015.06.131

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