Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

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Zhao, X., Moates, G. K., Elliston, A., Wilson, D. R., Coleman, M. and Waldron, K. W. (2015) Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre. Bioresource Technology, 194. 263–269. ISSN 1873-2976

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Abstract

This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using CellicÒ CTec 2 (20 U or 0.87 FPU gﰂ1 substrate) cellulase plus b-glucosidase (2 U gﰂ1 substrate) and a yeast inoculum of 10% (v/v or 8.0 ﰀ 107 cells mLﰂ1). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concen- tration of 20% (w/v) by metabolising fermentation inhibitors.

Item Type: Article
Additional Information: Copyright 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license
Uncontrolled Keywords: duckweed,bioethanol production,steam explosion,cellulase,simultaneous saccharification ,fermentation
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Plant Sciences
Faculty of Science > Research Groups > Biosciences Teaching and Education Research
Depositing User: Pure Connector
Date Deposited: 25 Aug 2015 09:20
Last Modified: 15 Oct 2025 18:30
URI: https://ueaeprints.uea.ac.uk/id/eprint/54118
DOI: 10.1016/j.biortech.2015.06.131

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