Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells

Wang, Yichong, Dacosta, Christopher, Wang, Wei, Zhou, Zhigang, Liu, Chun-Ming and Bao, Yongping (2015) Synergy between sulforaphane and selenium in protection against oxidative damage in colonic CCD841 cells. Nutrition Research, 35 (7). 610–617. ISSN 0271-5317

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Abstract

Dietary isothiocyanates (ITCs) are potent inducers of the NF-E2-related factor-2 (Nrf2) pathway. Sulforaphane (SFN), a representative ITC, has previously been shown to upregulate antioxidant enzymes such as selenium (Se) dependent thioredoxin reductase-1 (TrxR-1) in many tumour cell lines. In the present study, we hypothesized that a combination of SFN and Se would have a synergistic effect on the upregulation of TrxR-1 and the protection against oxidative damage in the normal colonic cell line CCD841. Treatment of cells with SFN and Se significantly induced TrxR-1 expression. Pre-treatment of cells with SFN protects against H2O2-induced cell death; this protection was enhanced by co-treatment with Se. The siRNA knockdown of either TrxR-1 or Nrf2 reduced the protection afforded by SFN and Se co-treatment; TrxR-1 and Nrf2 knockdown reduced cell viabilities to 66.5 and 51.1% respectively, down from 82.4% in transfection negative controls. This suggests that both TrxR-1 and Nrf2 are important in SFN-mediated protection against free radical-induced cell death. Moreover, flow cytometric analysis showed that TrxR-1 and Nrf2 were involved in SFN-mediated protection against H2O2-induced apoptosis. In summary, SFN activates the Nrf2 signaling pathway and protects against H2O2-mediated oxidative damage in normal colonic cells. Combined SFN and Se treatment resulted in a synergistic upregulation of TrxR-1 that in part contributed to the enhanced protection against free radical-mediated cell death provided by the co-treatment.

Item Type: Article
Uncontrolled Keywords: cruciferous vegetable,sulforaphane,selenium,thioredoxin reductase,nrf2,colon cancer
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 24 Jul 2015 22:48
Last Modified: 27 Jul 2020 23:45
URI: https://ueaeprints.uea.ac.uk/id/eprint/53705
DOI: 10.1016/j.nutres.2015.05.011

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