Pectins and pectic-oligosaccharides inhibit Escherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29

Olano-Martin, Estibaliz, Williams, Mark, Gibson, Glenn R and Rastall, Robert A (2003) Pectins and pectic-oligosaccharides inhibit Escherichia coliO157:H7 Shiga toxin as directed towards the human colonic cell line HT29. FEMS Microbiology Letters, 218 (1). pp. 101-105. ISSN 1574-6968

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Abstract

Pectins and pectic-oligosaccharides, as derived by controlled enzymatic hydrolysis, were evaluated for their ability to interfere with the toxicity of Shiga-like toxins from Escherichia coli O157:H7. Both types of material resulted in some degree of protection but this was significantly higher (P>0.01) with the oligosaccharide fractions (giving 90–100% cell survival, compared to 70–80% with the polymer). An effect of methylation on the protective effect was detected with lower degrees being more active. The pectic-oligosaccharides and galabiose, the minimum toxin receptor analogue, were shown to inhibit toxicity and were both protective at 10 mg ml−1, but not at lower concentrations.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:37
Last Modified:	24 Sep 2024 10:12
URI:	https://ueaeprints.uea.ac.uk/id/eprint/522
DOI:	10.1111/j.1574-6968.2003.tb11504.x

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