Wong, Steve, Grigg, Jason, Le Brun, Nick ORCID: https://orcid.org/0000-0001-9780-4061, Moore, Geoff, Murphy, Michael and Mauk, Grant
(2015)
The B-type channel is a major route for iron entry into the ferroxidase center and central cavity of bacterioferritin.
The Journal of Biological Chemistry, 290 (6).
pp. 3732-3739.
ISSN 1083-351X
Abstract
Bacterioferritin is a bacterial iron storage and detoxification protein that is capable of forming a ferric oxyhydroxide mineral core within its central cavity. To do this, iron must traverse the bacterioferritin protein shell, which is expected to occur through one or more of the channels through the shell identified by structural studies. The size and negative electrostatic potential of the 24 B-type channels suggest that they could provide a route for iron into bacterioferritin. Residues at the B-type channel (N34, E66, D132, and D139) of E. coli bacterioferritin were substituted to determine if they are important for iron core formation. A significant decrease in the rates of initial oxidation of Fe(II) at the ferroxidase center and subsequent iron mineralization was observed for the D132F variant. The crystal structure of this variant shows that substitution of residue 132 with phenylalanine caused a steric blockage of the B-type channel and no other material structural perturbation. We conclude that the B-type channel is a major route for iron entry into both the ferroxidase center and the iron storage cavity of bacterioferritin.
Item Type: | Article |
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Additional Information: | Open Access with a Creative Commons Attribution Unported Licence |
Uncontrolled Keywords: | escherichia coli (e. coli),ferritin,iron,site-directed mutagenesis,x-ray crystallography,bacterioferritin,iron storage,iron core |
Faculty \ School: | Faculty of Science > School of Chemistry |
UEA Research Groups: | Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes |
Depositing User: | Pure Connector |
Date Deposited: | 02 Feb 2015 15:52 |
Last Modified: | 04 Mar 2024 17:23 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/52073 |
DOI: | 10.1074/jbc.M114.623082 |
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