Combinational electroporation and transplantation approach of studying gene functions in avian embryos

Wang, Xiaoyu, Li, Yan, Wang, Guang, Münsterberg, Andrea ORCID: https://orcid.org/0000-0002-4577-4240, Chuai, Manli, Lee, Kenneth, Wang, Lijing and Yang, Xuesong (2014) Combinational electroporation and transplantation approach of studying gene functions in avian embryos. Chinese Science Bulletin, 59 (7). pp. 616-624. ISSN 1861-9541

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Abstract

Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying biological function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to understanding how genes regulate embryo development. Furthermore, combining these approaches with tissue transplant techniques make even more attractive for elucidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned. We presently demonstrated the different patterns of organizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically transfect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.

Item Type:	Article
Additional Information:	Funding information: This work was supported by the National Natural Science Foundation of China (31071054, 30971493, 31271455), the National Basic Research Program of China (2010CB529703) and the Natural Science Foundation of Guangdong Province (S2011010001593).
Uncontrolled Keywords:	chick embryo,electroporation,gene transfection,transplantation
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Cells and Tissues
Depositing User:	Pure Connector
Date Deposited:	02 Jan 2015 12:00
Last Modified:	01 Oct 2024 15:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/51592
DOI:	10.1007/s11434-013-0090-8

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