The clinical utility of Multiplate analyser measurement in platelet function testing following stroke and transient ischaemic attack

Mannu, Gurdeep S., Macartney, Alistair, Lambert, Jonathan R. A., Bettencourt-silva, Joao H., Lawn, Mark, Lyall, Hamish, Metcalf, Anthony Kneale, Potter, John F., Wood, John, Clark, Allan ORCID: https://orcid.org/0000-0003-2965-8941, Baglin, Trevor, Myint, Phyo Kyaw and Bowles, Kristian M. ORCID: https://orcid.org/0000-0003-1334-4526 (2015) The clinical utility of Multiplate analyser measurement in platelet function testing following stroke and transient ischaemic attack. European Journal of Haematology, 94 (2). 138–144. ISSN 0902-4441

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Abstract

Background: Platelet responsiveness to aspirin in people with cerebrovascular disease is poorly understood. Objectives: To determine: (i) normal reference range, imprecision and reproducibility of the Multiplate instrument in healthy volunteers naive to aspirin; (ii) imprecision and reproducibility of the Multiplate instrument in acute stroke and transient ischaemic attack (TIA); (iii) the relationship between aspirin responsiveness and clinical outcome. Materials and Methods: We evaluated platelet function response to three agonists [Adenosine Diphosphate (ADP), Arachidonic Acid (AA), Collagen (Col)] using the Mulitplate platelet function analyser in a two-phase pilot study. In phase 1, we recruited healthy volunteers to determine the normal reference range and imprecision of the Multiplate instrument. In phase 2, we assessed platelet function in acute stroke or TIA patients presenting to hospital. These patients were bled within 24 h of presentation and between 12 and 24 h after ≥75 mg dose of Aspirin. Patients were followed up to 1 yr to assess mortality and recurrent cardiovascular event. Results: Overall, 29 healthy volunteers and 81 stroke/TIA patients were recruited. On assessing components of variance, Multiplate testing is reproducible and precise in volunteers and stroke/TIA patients. In stroke patients receiving aspirin, Bland–Altman plots show initial day 1 measurement provided a reliable measure of continuing response to aspirin at day 3. We defined one-third of patients as aspirin resistant [31.8% (95% CI: 22.1%–42.8%)] using cut off mean aggregation of ≥23.08% for AA and mean aggregation of ≥80.76% for ADP. Conclusion: The Multiplate device gives reproducible, precise results in volunteers and stroke/TIA patients.

Item Type:	Article
Uncontrolled Keywords:	aspirin resistance,platelet function testing,stroke,transient ischaemic attack,prognosis
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Norwich Clinical Trials Unit Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Groups > Health Services and Primary Care Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023) Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Population Health
Depositing User:	Pure Connector
Date Deposited:	12 Nov 2014 16:46
Last Modified:	19 Oct 2023 01:24
URI:	https://ueaeprints.uea.ac.uk/id/eprint/50845
DOI:	10.1111/ejh.12406

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