Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni

Brown, Helen, Reuter, Mark, Cross, Kathryn, Salt, Louise, Betts, Roy and Van Vliet, Arnoud (2014) Chicken juice enhances surface attachment and biofilm formation of Campylobacter jejuni. Applied and Environmental Microbiology, 80 (22). pp. 7053-7060. ISSN 0099-2240

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Abstract

The bacterial pathogen Campylobacter jejuni is primarily transmitted via the consumption of contaminated food-stuffs, especially poultry meat. In food processing environments, C. jejuni is required to survive a multitude of stresses and requires the use of specific survival mechanisms, such as biofilms. An initial step in biofilm formation is bacterial attachment to a surface. Here we have investigated the effects of a chicken meat exudate (chicken juice) on C. jejuni surface attachment and biofilm formation. Supplementation of Brucella broth with ≥5% chicken juice resulted in increased biofilm formation on glass, polystyrene and stainless steel surfaces with four C. jejuni isolates and one C. coli isolate, in both microaerobic and aerobic conditions. When incubated with chicken juice, C. jejuni was both able to grow, and form biofilms in static cultures in aerobic conditions. Electron microscopy showed that C. jejuni cells were associated with chicken juice particulates attached to the abiotic surface, rather than the surface itself. This suggests chicken juice contributes to C. jejuni biofilm formation by covering and conditioning the abiotic surface, and is a source of nutrients. Chicken juice was able to complement the reduction in biofilm formation of an aflagellated mutant of C. jejuni, indicating that chicken juice may support food chain transmission of isolates with lowered motility. This work provides a useful model for studying the interaction of C. jejuni biofilms in food chain-relevant conditions, and also shows a possible mechanism for C. jejuni cell attachment and biofilm initiation on abiotic surfaces within the food chain.

Item Type:	Article
Additional Information:	Copyright © 2014 Brown et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.
Uncontrolled Keywords:	campylobacter jejuni,biofilm,chicken exudate,surface conditioning,food safety
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User:	Pure Connector
Date Deposited:	31 Oct 2014 13:26
Last Modified:	20 Oct 2022 20:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/50499
DOI:	10.1128/AEM.02614-14

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