In vitro and in vivo studies of trypanocidal activity of dietary isothiocyanates

Steverding, Dietmar, Michaels, Sarah and Read, Kevin D. (2014) In vitro and in vivo studies of trypanocidal activity of dietary isothiocyanates. Planta Medica, 80 (2-3). pp. 183-186. ISSN 0032-0943

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Six dietary isothiocyanates, allyl-isothiocyanate, benzyl-isothiocyanate, phenylethyl-isothiocyanate, sulforaphane, erucin, and iberin, were tested for their trypanocidal activities in vitro using culture-adapted bloodstream forms of Trypanosoma brucei. All isothiocyanates showed a dose-dependent effect on the growth of trypanosomes. Five compounds displayed MIC values of 10 μM and GI values of around 1.5 μM, while allyl-isothiocyanate exhibited values of 100 and 11 μM, respectively. The compounds showed similar cytotoxic activities against human HL-60 cells with GI values of 1-4 μM and MIC values of 10-100 μM. Short-term experiments revealed that, with the exception of allyl-isothiocyanate, isothiocyanates at a concentration of 10 μM kill trypanosomes within 1-4 h of incubation. In contrast, HL-60 cells were not affected by any of the compounds in short-term incubation experiments. Sulforaphane, the most intensively studied isothiocyanate, was also investigated for its in vivo trypanocidal activity. However, administration of 50 mg/kg sulforaphane orally or intraperitoneally for four days had no effect on the parasitaemia in mice infected with T. brucei compared to control animals treated with vehicle alone.

Item Type: Article
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
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Depositing User: Pure Connector
Date Deposited: 23 Sep 2014 14:18
Last Modified: 21 Oct 2022 00:09
DOI: 10.1055/s-0033-1360262


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