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ToolsKrüssel, Lena, Junemann, Johannes, Wirtz, Markus, Birke, Hannah, Thornton, Jeremy D., Browning, Luke W., Poschet, Gernot, Hell, Rüdiger, Balk, Janneke, Braun, Hans-Peter and Hildebrandt, Tatjana (2014) The mitochondrial sulfur dioxygenase ETHE1 is required for amino acid catabolism during carbohydrate starvation and embryo development in Arabidopsis thaliana. Plant Physiology, 165 (1). pp. 92-104. ISSN 0032-0889
Full text not available from this repository. (Request a copy)Abstract
The sulfur dioxygenase ETHE1 catalyzes the oxidation of persulfides in the mitochondrial matrix and is essential for early embryo development in Arabidopsis thaliana. We investigated the biochemical and physiological function of ETHE1 in plant metabolism using recombinant Arabidopsis ETHE1 and three T-DNA insertion lines with 50-99 % decreased sulfur dioxygenase activity. Our results identified a new mitochondrial pathway catalyzing the detoxification of reduced sulfur species derived from cysteine catabolism by oxidation to thiosulfate. Knockdown of the sulfur dioxygenase impaired embryo development and produced phenotypes of starvation-induced chlorosis during short-day growth condition and extended darkness, indicating that ETHE1 has a key function in situations of high protein turnover such as seed production and the use of amino acids as alternative respiratory substrates during carbohydrate starvation. The amino acid profile of mutant plants was similar to that caused by defects in the electron-transfer flavoprotein/electron-transfer flavoprotein:ubiquinone oxidoreductase complex and associated dehydrogenases. Thus, in addition to sulfur amino acid catabolism ETHE1 also affects the oxidation of branched-chain amino acids and lysine.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | Pure Connector |
| Date Deposited: | 19 Aug 2014 09:06 |
| Last Modified: | 01 Nov 2022 10:18 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/49930 |
| DOI: | 10.1104/pp.114.239764 |
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