Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas

Chiodo, Fabrizio, Enriquez-Navas, Pedro M., Angulo, Jesus ORCID: https://orcid.org/0000-0001-7250-5639, Marradi, Marco and Penadés, Soledad (2015) Assembling different antennas of the gp120 high mannose-type glycans on gold nanoparticles provides superior binding to the anti-HIV antibody 2G12 than the individual antennas. Carbohydrate Research, 405. pp. 102-109. ISSN 0008-6215

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Abstract

In order to re-build Man9GlcNAc2 clusters of the HIV gp120 glycoprotein, ~2 nm gold glyconanoparticles (GNPs) were coated with the synthetic partial structures of Man9, the tetramannoside Manα1-2Manα1-2Manα1-3Manα1- and the pentamannoside Manα1–2Manα1–3[Manα1–2Manα1–6]Manα1-. Their interactions with the anti-HIV broadly neutralizing antibody 2G12 were studied by Surface Plasmon Resonance (SPR)-based biosensors and saturation transfer difference (STD)-NMR spectroscopy. A synergistic effect of the tetra- and pentamannosides multimerised on a same GNP was observed. The assembly of these antennas of the gp120 high-mannose type glycan on GNPs provided superior binding to the anti-HIV antibody 2G12 respect to GNPs carrying only the individual oligomannosides. The results presented in this work provide new molecular information on the interactions between clusters of oligomannosides and 2G12 that could help in the design of a carbohydrate-based vaccine against HIV.

Item Type:	Article
Uncontrolled Keywords:	gold glyconanoparticles,high-mannose type glycans,2g12,binding studies,surface plasmon resonance,saturation transfer difference nmr spectroscopy,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	25 Jul 2014 15:24
Last Modified:	25 Sep 2024 11:25
URI:	https://ueaeprints.uea.ac.uk/id/eprint/49720
DOI:	10.1016/j.carres.2014.07.012

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