Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans

Day, Richard M., Harbord, Marcus, Forbes, Alastair ORCID: https://orcid.org/0000-0001-7416-9843 and Segal, Anthony W. (2001) Cantharidin blisters: a technique for investigating leukocyte trafficking and cytokine production at sites of inflammation in humans. Journal of Immunological Methods, 257 (1-2). pp. 213-220. ISSN 0022-1759

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Abstract

A skin blister technique is described which allows the investigation of acute inflammation in humans in vivo. Filter paper discs are placed on the skin, impregnated with cantharidin and covered with impermeable film held by adhesive tape. The assembly is easily applied, unobtrusive, stable and may be worn during normal activities. The blister formed at 24 h contains approximately 5x10(5)-5x10(6) cells, predominantly neutrophils and macrophages. Inflammatory cytokines and chemotactic factors are detectable in the blister fluid. The technique is useful for characterizing the acute inflammatory response in health and disease.

Item Type:	Article
Uncontrolled Keywords:	blister,body fluids,cantharidin,cell movement,cytokines,dermatitis, contact,humans,inflammation,irritants,leukocytes
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology
Depositing User:	Pure Connector
Date Deposited:	06 Aug 2014 10:40
Last Modified:	20 Oct 2022 23:58
URI:	https://ueaeprints.uea.ac.uk/id/eprint/49577
DOI:	10.1016/S0022-1759(01)00467-7

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