An enzyme-linked immunosorbent assay for fetal steroid binding protein of human serum

Iqbal, M J, Forbes, A ORCID:, Corbishley, T P, Wilkinson, M L and Williams, R (1985) An enzyme-linked immunosorbent assay for fetal steroid binding protein of human serum. Steroids, 45 (1). pp. 31-8. ISSN 0039-128X

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The binding characteristics and quantitation of the recently reported fetal steroid binding protein (FSBP) cannot be determined on unpurified samples; an immunoassay was therefore desirable. The protein was purified to homogeneity in order to raise a highly specific polyclonal antibody. An enzyme-linked immunosorbent assay applicable to unpurified samples was developed. Intra- and inter-assay coefficients of variation are 8.0% and 9.2% respectively; there is a sensitivity of 30 fmol FSBP per well, and there is no cross-reactivity with other binding proteins. Results obtained with the assay correlate with the more complex ligand binding assay (r = 0.85; p less than 0.02). Measurement of sera showed that FSBP levels are higher in women than in men (51.2 +/- 10.62 nM; 41.2 +/- 13.65 respectively; p less than 0.05) and are elevated in cirrhotic women (66.4 +/- 18.67; p less than 0.05) and in males with hepatocellular carcinoma (62.2 +/- 13.05; p less than 0.002). Use of the enzyme-linked immunosorbent assay confirmed the identity of FSBP separate from sex hormone binding globulin.

Item Type: Article
Uncontrolled Keywords: carcinoma, hepatocellular,carrier proteins,enzyme-linked immunosorbent assay,female,fetus,humans,liver cirrhosis,liver neoplasms,male,pregnancy,quality control,sex hormone-binding globulin,steroids
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 06 Aug 2014 10:46
Last Modified: 24 Oct 2022 23:58

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