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Iqbal, M J, Forbes, A 
ORCID: https://orcid.org/0000-0001-7416-9843, Corbishley, T P, Wilkinson, M L and Williams, R
(1985)
An enzyme-linked immunosorbent assay for fetal steroid binding protein of human serum.
Steroids, 45 (1).
pp. 31-8.
ISSN 0039-128X
Abstract
The binding characteristics and quantitation of the recently reported fetal steroid binding protein (FSBP) cannot be determined on unpurified samples; an immunoassay was therefore desirable. The protein was purified to homogeneity in order to raise a highly specific polyclonal antibody. An enzyme-linked immunosorbent assay applicable to unpurified samples was developed. Intra- and inter-assay coefficients of variation are 8.0% and 9.2% respectively; there is a sensitivity of 30 fmol FSBP per well, and there is no cross-reactivity with other binding proteins. Results obtained with the assay correlate with the more complex ligand binding assay (r = 0.85; p less than 0.02). Measurement of sera showed that FSBP levels are higher in women than in men (51.2 +/- 10.62 nM; 41.2 +/- 13.65 respectively; p less than 0.05) and are elevated in cirrhotic women (66.4 +/- 18.67; p less than 0.05) and in males with hepatocellular carcinoma (62.2 +/- 13.05; p less than 0.002). Use of the enzyme-linked immunosorbent assay confirmed the identity of FSBP separate from sex hormone binding globulin.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | carcinoma, hepatocellular,carrier proteins,enzyme-linked immunosorbent assay,female,fetus,humans,liver cirrhosis,liver neoplasms,male,pregnancy,quality control,sex hormone-binding globulin,steroids |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine |
| Depositing User: | Pure Connector |
| Date Deposited: | 06 Aug 2014 10:46 |
| Last Modified: | 24 Oct 2022 23:58 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/49497 |
| DOI: |
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