Mechanism of [4Fe-4S](Cys)4 cluster nitrosylation is conserved among NO-responsive regulators

Crack, Jason C, Stapleton, Melanie R, Green, Jeffrey, Thomson, Andrew J and Le Brun, Nick E ORCID: https://orcid.org/0000-0001-9780-4061 (2013) Mechanism of [4Fe-4S](Cys)4 cluster nitrosylation is conserved among NO-responsive regulators. The Journal of Biological Chemistry, 288 (16). pp. 11492-502. ISSN 1083-351X

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Abstract

The Fumarate nitrate reduction (FNR) regulator from Escherichia coli controls expression of >300 genes in response to O2 through reaction with its [4Fe-4S] cluster cofactor. FNR is the master switch for the transition between anaerobic and aerobic respiration. In response to physiological concentrations of nitric oxide (NO), FNR also regulates genes, including the nitrate reductase (nar) operon, a major source of endogenous cellular NO, and hmp, which encodes an NO-detoxifying enzyme. Here we show that the [4Fe-4S] cluster of FNR reacts rapidly in a multiphasic reaction with eight NO molecules. Oxidation of cluster sulfide ions (S(2-)) to sulfane (S(0)) occurs, some of which remains associated with the protein as Cys persulfide. The nitrosylation products are similar to a pair of dinuclear dinitrosyl iron complexes, [Fe(I)2(NO)4(Cys)2](0), known as Roussin's red ester. A similar reactivity with NO was reported for the Wbl family of [4Fe-4S]-containing proteins found only in actinomycetes, such as Streptomyces and Mycobacteria. These results show that NO reacts via a common mechanism with [4Fe-4S] clusters in phylogenetically unrelated regulatory proteins that, although coordinated by four Cys residues, have different cluster environments. The reactivity of E. coli FNR toward NO, in addition to its sensitivity toward O2, is part of a hierarchal network that monitors, and responds to, NO, both endogenously generated and exogenously derived.

Item Type:	Article
Uncontrolled Keywords:	aerobiosis,anaerobiosis,escherichia coli,escherichia coli proteins,iron,iron-sulfur proteins,mycobacterium,nitric oxide,operon,oxygen,streptomyces,sulfides
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Centre for Ocean and Atmospheric Sciences Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes
Depositing User:	Pure Connector
Date Deposited:	09 Jul 2014 10:14
Last Modified:	25 Sep 2024 11:19
URI:	https://ueaeprints.uea.ac.uk/id/eprint/49066
DOI:	10.1074/jbc.M112.439901

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