Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation

Zipfel, Cyril, Kunze, Gernot, Chinchilla, Delphine, Caniard, Anne, Jones, Jonathan D G, Boller, Thomas and Felix, Georg (2006) Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation. Cell, 125 (4). pp. 749-60. ISSN 0092-8674

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Abstract

Higher eukaryotes sense microbes through the perception of pathogen-associated molecular patterns (PAMPs). Arabidopsis plants detect a variety of PAMPs including conserved domains of bacterial flagellin and of bacterial EF-Tu. Here, we show that flagellin and EF-Tu activate a common set of signaling events and defense responses but without clear synergistic effects. Treatment with either PAMP results in increased binding sites for both PAMPs. We used this finding in a targeted reverse-genetic approach to identify a receptor kinase essential for EF-Tu perception, which we called EFR. Nicotiana benthamiana, a plant unable to perceive EF-Tu, acquires EF-Tu binding sites and responsiveness upon transient expression of EFR. Arabidopsis efr mutants show enhanced susceptibility to the bacterium Agrobacterium tumefaciens, as revealed by a higher efficiency of T-DNA transformation. These results demonstrate that EFR is the EF-Tu receptor and that plant defense responses induced by PAMPs such as EF-Tu reduce transformation by Agrobacterium.

Item Type: Article
Uncontrolled Keywords: agrobacterium tumefaciens,amino acid sequence,arabidopsis,arabidopsis proteins,bacterial proteins,binding sites,flagellin,gene expression regulation, plant,molecular sequence data,mutation,peptide elongation factor tu,protein kinases,receptors, cell surface,sequence alignment,signal transduction,tobacco,transcription, genetic,transformation, genetic
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 09 Jul 2014 12:22
Last Modified: 24 Jul 2019 20:00
URI: https://ueaeprints.uea.ac.uk/id/eprint/48891
DOI: 10.1016/j.cell.2006.03.037

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