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ToolsZipfel, Cyril, Kunze, Gernot, Chinchilla, Delphine, Caniard, Anne, Jones, Jonathan D G, Boller, Thomas and Felix, Georg (2006) Perception of the bacterial PAMP EF-Tu by the receptor EFR restricts Agrobacterium-mediated transformation. Cell, 125 (4). pp. 749-60. ISSN 0092-8674
Full text not available from this repository. (Request a copy)Abstract
Higher eukaryotes sense microbes through the perception of pathogen-associated molecular patterns (PAMPs). Arabidopsis plants detect a variety of PAMPs including conserved domains of bacterial flagellin and of bacterial EF-Tu. Here, we show that flagellin and EF-Tu activate a common set of signaling events and defense responses but without clear synergistic effects. Treatment with either PAMP results in increased binding sites for both PAMPs. We used this finding in a targeted reverse-genetic approach to identify a receptor kinase essential for EF-Tu perception, which we called EFR. Nicotiana benthamiana, a plant unable to perceive EF-Tu, acquires EF-Tu binding sites and responsiveness upon transient expression of EFR. Arabidopsis efr mutants show enhanced susceptibility to the bacterium Agrobacterium tumefaciens, as revealed by a higher efficiency of T-DNA transformation. These results demonstrate that EFR is the EF-Tu receptor and that plant defense responses induced by PAMPs such as EF-Tu reduce transformation by Agrobacterium.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | agrobacterium tumefaciens,amino acid sequence,arabidopsis,arabidopsis proteins,bacterial proteins,binding sites,flagellin,gene expression regulation, plant,molecular sequence data,mutation,peptide elongation factor tu,protein kinases,receptors, cell surface,sequence alignment,signal transduction,tobacco,transcription, genetic,transformation, genetic |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Depositing User: | Pure Connector |
| Date Deposited: | 09 Jul 2014 12:22 |
| Last Modified: | 24 Oct 2022 06:25 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/48891 |
| DOI: | 10.1016/j.cell.2006.03.037 |
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