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ToolsLiebminger, Eva, Veit, Christiane, Pabst, Martin, Batoux, Martine, Zipfel, Cyril, Altmann, Friedrich, Mach, Lukas and Strasser, Richard (2011) Beta-N-acetylhexosaminidases HEXO1 and HEXO3 are responsible for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. The Journal of Biological Chemistry, 286 (12). pp. 10793-10802. ISSN 1083-351X
Full text not available from this repository.Abstract
Most plant glycoproteins contain substantial amounts of paucimannosidic N-glycans instead of their direct biosynthetic precursors, complex N-glycans with terminal N-acetylglucosamine residues. We now demonstrate that two β-N-acetylhexosaminidases (HEXO1 and HEXO3) residing in different subcellular compartments jointly account for the formation of paucimannosidic N-glycans in Arabidopsis thaliana. Total N-glycan analysis of hexo knock-out plants revealed that HEXO1 and HEXO3 contribute equally to the production of paucimannosidic N-glycans in roots, whereas N-glycan processing in leaves depends more heavily on HEXO3 than on HEXO1. Because hexo1 hexo3 double mutants do not display any obvious phenotype even upon exposure to different forms of abiotic or biotic stress, it should be feasible to improve the quality of glycoprotein therapeutics produced in plants by down-regulation of endogenous β-N-acetylhexosaminidase activities.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | arabidopsis,arabidopsis proteins,gene knockdown techniques,plant roots,plants, genetically modified,polysaccharides,stress, physiological,beta-n-acetylhexosaminidases |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Depositing User: | Pure Connector |
| Date Deposited: | 09 Jul 2014 12:14 |
| Last Modified: | 24 Oct 2022 06:25 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/48879 |
| DOI: | 10.1074/jbc.M110.178020 |
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