Ntoukakis, Vardis, Schwessinger, Benjamin, Segonzac, Cécile and Zipfel, Cyril (2011) Cautionary notes on the use of C-terminal BAK1 fusion proteins for functional studies. The Plant Cell, 23 (11). pp. 3871-3878. ISSN 1040-4651
Full text not available from this repository.Abstract
Detailed phenotypic characterization reveals that several BAK1 fusion proteins with C-terminal tags strongly impair complementation of bak1 null mutants with respect to responsiveness to the bacterial pathogen-associated molecular patterns flagellin and EF-Tu. This raises concerns about the widespread use of such protein variants of this important regulatory Leu-rich repeat receptor-like kinase (RLK) for functional analyses of RLK-based signaling.
Item Type: | Article |
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Uncontrolled Keywords: | arabidopsis,arabidopsis proteins,brassinosteroids,flagellin,mutation,peptide elongation factor tu,plants, genetically modified,protein kinases,protein-serine-threonine kinases,recombinant fusion proteins,signal transduction |
Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory |
UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
Depositing User: | Pure Connector |
Date Deposited: | 04 Jul 2014 12:44 |
Last Modified: | 24 Oct 2022 06:25 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/48875 |
DOI: | 10.1105/tpc.111.090779 |
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