Regulation of CD3 expression in a protein kinase C isozyme-deficient T-cell line

Long, A, O'Connell, M ORCID:, Liskamp, R M and Kelleher, D (1993) Regulation of CD3 expression in a protein kinase C isozyme-deficient T-cell line. Immunology, 80 (4). pp. 654-657. ISSN 0019-2805

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Using the T-cell lymphoma line HuT 78, and a clone derived from HuT 78, designated K-4, the role of protein kinase C (PKC) isozymes in the expression of a variety of T-cell surface antigens was investigated. HuT 78 expresses PKC isozymes alpha and beta while K-4 expresses only PKC alpha. Flow cytometric analysis revealed that incubation of HuT 78 cells with phorbol 12-myristate 13-acetate (PMA) results in significant down-regulation of surface expression of CD3. While K-4 cells expressed reduced amounts of CD3, a similar reduction in CD3 expression was not observed when these cells were stimulated with PMA. The regulation of expression of CD11a (LFA-1), CD44, CD45RA and CD45RO and of the class II molecules DR and DP in response to PMA, was similar in both cell lines.

Item Type: Article
Uncontrolled Keywords: antigens, cd3,antigens, surface,humans,isoenzymes,protein kinase c,t-lymphocytes,tetradecanoylphorbol acetate,tumor cells, cultured
Faculty \ School: Faculty of Science > School of Pharmacy
UEA Research Groups: Faculty of Science > Research Groups > Pharmaceutical Cell Biology (former - to 2017)
Faculty of Science > Research Groups > Molecular and Tissue Pharmacology
Depositing User: Pure Connector
Date Deposited: 11 Jun 2014 13:10
Last Modified: 24 Oct 2022 06:14

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