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Long, A, O'Connell, M 
ORCID: https://orcid.org/0000-0002-0267-0951, Liskamp, R M and Kelleher, D
(1993)
Regulation of CD3 expression in a protein kinase C isozyme-deficient T-cell line.
Immunology, 80 (4).
pp. 654-657.
ISSN 0019-2805
Abstract
Using the T-cell lymphoma line HuT 78, and a clone derived from HuT 78, designated K-4, the role of protein kinase C (PKC) isozymes in the expression of a variety of T-cell surface antigens was investigated. HuT 78 expresses PKC isozymes alpha and beta while K-4 expresses only PKC alpha. Flow cytometric analysis revealed that incubation of HuT 78 cells with phorbol 12-myristate 13-acetate (PMA) results in significant down-regulation of surface expression of CD3. While K-4 cells expressed reduced amounts of CD3, a similar reduction in CD3 expression was not observed when these cells were stimulated with PMA. The regulation of expression of CD11a (LFA-1), CD44, CD45RA and CD45RO and of the class II molecules DR and DP in response to PMA, was similar in both cell lines.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | antigens, cd3,antigens, surface,humans,isoenzymes,protein kinase c,t-lymphocytes,tetradecanoylphorbol acetate,tumor cells, cultured |
| Faculty \ School: | Faculty of Science > School of Pharmacy (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Pharmaceutical Cell Biology (former - to 2017) Faculty of Science > Research Groups > Molecular and Tissue Pharmacology |
| Depositing User: | Pure Connector |
| Date Deposited: | 11 Jun 2014 13:10 |
| Last Modified: | 25 Sep 2024 11:13 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/48276 |
| DOI: |
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