Conjugated linoleic acid alters global gene expression in human intestinal-like Caco-2 cells in an isomer-specific manner

Murphy, Eileen F, Hooiveld, Guido J, Muller, Michael, Calogero, Raffaele A and Cashman, Kevin D (2007) Conjugated linoleic acid alters global gene expression in human intestinal-like Caco-2 cells in an isomer-specific manner. Journal of Nutrition, 137 (11). pp. 2359-2365. ISSN 0022-3166

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Abstract

Conjugated linoleic acid (CLA) exhibits isomer-specific effects on transepithelial calcium (Ca) transport as well as on cell growth in human intestinal-like Caco-2 cells. However, the molecular mechanisms of action are still unclear. Therefore, this study used a transcriptomic approach to help elucidate the molecular mechanisms underlying such isomer-specific effects. Caco-2 cells were treated with 80 micromol/L linoleic acid (control), 80 micromol/L trans-10, cis-12 CLA, or 80 micromol/L cis-9, trans-11 CLA for 12 d. Ca transport was measured radio-isotopically. RNA was isolated from the cells, labeled, and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis was preformed using Bioconductor. Using a minimum fold-change criterion of 1.6 and a false discovery rate criterion of P-value

Item Type: Article
Uncontrolled Keywords: adenocarcinoma,calcium,tumor cell line,colonic neoplasms,computational biology,gene expression profiling,gene expression regulation,gene expression regulation, neoplastic,humans,intestinal mucosa,conjugated ,oligonucleotide array sequence analysis,neoplasm rna,structure-activity relationship
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: Pure Connector
Date Deposited: 03 Mar 2014 18:10
Last Modified: 21 Apr 2020 22:53
URI: https://ueaeprints.uea.ac.uk/id/eprint/47718
DOI: 10.1093/jn/137.11.2359

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