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Murphy, Eileen F, Hooiveld, Guido J, Muller, Michael 
ORCID: https://orcid.org/0000-0002-5930-9905, Calogero, Raffaele A and Cashman, Kevin D
(2007)
Conjugated linoleic acid alters global gene expression in human intestinal-like Caco-2 cells in an isomer-specific manner.
Journal of Nutrition, 137 (11).
pp. 2359-2365.
ISSN 0022-3166
Abstract
Conjugated linoleic acid (CLA) exhibits isomer-specific effects on transepithelial calcium (Ca) transport as well as on cell growth in human intestinal-like Caco-2 cells. However, the molecular mechanisms of action are still unclear. Therefore, this study used a transcriptomic approach to help elucidate the molecular mechanisms underlying such isomer-specific effects. Caco-2 cells were treated with 80 micromol/L linoleic acid (control), 80 micromol/L trans-10, cis-12 CLA, or 80 micromol/L cis-9, trans-11 CLA for 12 d. Ca transport was measured radio-isotopically. RNA was isolated from the cells, labeled, and hybridized to the Affymetrix U133 2.0 Plus arrays (n = 3). Data and functional analysis was preformed using Bioconductor. Using a minimum fold-change criterion of 1.6 and a false discovery rate criterion of P-value
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | adenocarcinoma,calcium,tumor cell line,colonic neoplasms,computational biology,gene expression profiling,gene expression regulation,gene expression regulation, neoplastic,humans,intestinal mucosa,conjugated ,oligonucleotide array sequence analysis,neoplasm rna,structure-activity relationship |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Depositing User: | Pure Connector |
| Date Deposited: | 03 Mar 2014 18:10 |
| Last Modified: | 06 Jun 2024 14:47 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/47718 |
| DOI: | 10.1093/jn/137.11.2359 |
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