Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice

de Wilde, Janneke, Mohren, Ronny, van den Berg, Sjoerd, Boekschoten, Mark, Dijk, Ko Willems-Van, de Groot, Philip, Müller, Michael ORCID: https://orcid.org/0000-0002-5930-9905, Mariman, Edwin and Smit, Egbert (2008) Short-term high fat-feeding results in morphological and metabolic adaptations in the skeletal muscle of C57BL/6J mice. Physiological Genomics, 32 (3). pp. 360-369. ISSN 1094-8341

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Abstract

The prevalence of the metabolic syndrome (MS) is rapidly increasing all over the world. Consequently, there is an urgent need for more effective intervention strategies. Both animal and human studies indicate that lipid oversupply to skeletal muscle can result in insulin resistance, which is one of the characteristics of the MS. C57BL/6J mice were fed a low-fat (10 kcal%) palm oil diet or a high-fat (45 kcal%; HF) palm oil diet for 3 or 28 days. By combining transcriptomics with protein and lipid analyses we aimed to better understand the molecular events underlying the early onset of the MS. Short-term HF feeding led to altered expression levels of genes involved in a variety of biological processes including morphogenesis, energy metabolism, lipogenesis, and immune function. Protein analysis showed increased levels of the myosin heavy chain, slow fiber type protein, and the complexes I, II, III, IV, and V of the oxidative phosphorylation. Furthermore, we observed that the main mitochondrial membrane phospholipids, phosphatidylcholine and phosphatidylethanolamine, contained more saturated fatty acids. Altogether, these results point to a morphological as well as a metabolic adaptation by promoting a more oxidative fiber type. We hypothesize that after this early positive adaptation, a continued transcriptional downregulation of genes involved in oxidative phosphorylation will result in decreased oxidative capacity at a later stage. Together with increased saturation of phospholipids of the mitochondrial membrane this can result in decreased mitochondrial function, which is a hallmark observed in insulin resistance and Type 2 diabetes.

Item Type:	Article
Uncontrolled Keywords:	adaptation, physiological,animals,blood glucose,dietary fats, unsaturated,fatty acids,gene expression profiling,gene expression regulation,glucose,insulin,male,metabolic syndrome x,mice,mice, inbred c57bl,muscle fibers, slow-twitch,muscle proteins,muscle, skeletal,oligonucleotide array sequence analysis,oxidation-reduction,plant oils,random allocation,trans-activators,transcription factors,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	10 Jun 2014 21:50
Last Modified:	06 Jun 2024 14:47
URI:	https://ueaeprints.uea.ac.uk/id/eprint/47710
DOI:	10.1152/physiolgenomics.00219.2007

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