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Haenen, Daniëlle, Zhang, Jing, Souza da Silva, Carol, Bosch, Guido, van der Meer, Ingrid M, van Arkel, Jeroen, van den Borne, Joost J G C, Pérez Gutiérrez, Odette, Smidt, Hauke, Kemp, Bas, Müller, Michael 
ORCID: https://orcid.org/0000-0002-5930-9905 and Hooiveld, Guido J E J
(2013)
A diet high in resistant starch modulates microbiota composition, SCFA concentrations, and gene expression in pig intestine.
Journal of Nutrition, 143 (3).
pp. 274-283.
ISSN 0022-3166
Abstract
Resistant starch (RS) is highly fermentable by microbiota in the colon, resulting in the production of SCFAs. RS is thought to mediate a large proportion of its health benefits, including increased satiety, through the actions of SCFAs. The aim of this study was to investigate the effects of a diet high in RS on luminal microbiota composition, luminal SCFA concentrations, and the expression of host genes involved in SCFA uptake, SCFA signaling, and satiety regulation in mucosal tissue obtained from small intestine, cecum, and colon. Twenty adult female pigs were either assigned to a digestible starch (DS) diet or a diet high in RS (34%) for a period of 2 wk. After the intervention, luminal content and mucosal scrapings were obtained for detailed molecular analysis. RS was completely degraded in the cecum. In both the cecum and colon, differences in microbiota composition were observed between DS- and RS-fed pigs. In the colon these included the stimulation of the healthy gut-associated butyrate-producing Faecalibacterium prausnitzii, whereas potentially pathogenic members of the Gammaproteobacteria, including Escherichia coli and Pseudomonas spp., were reduced in relative abundance. Cecal and colonic SCFA concentrations were significantly greater in RS-fed pigs, and cecal gene expression of monocarboxylate transporter 1 (SLC16A1) and glucagon (GCG) was induced by RS. In conclusion, our data show that RS modulates microbiota composition, SCFA concentrations, and host gene expression in pig intestine. Combined, our data provide an enhanced understanding of the interaction between diet, microbiota, and host.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | animals,bacteria,cecum,colon,diet,dietary carbohydrates,fatty acids, volatile,female,gene expression,glucagon,intestinal mucosa,intestine, large,lipid metabolism,metagenome,monocarboxylic acid transporters,starch,swine |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Depositing User: | Pure Connector |
| Date Deposited: | 21 Feb 2014 11:20 |
| Last Modified: | 06 Jun 2024 14:46 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/47649 |
| DOI: | 10.3945/jn.112.169672 |
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