Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor γ

Alex, Sheril, Lange, Katja, Amolo, Tom, Grinstead, Jeffrey S, Haakonsson, Anders K, Szalowska, Ewa, Koppen, Arjen, Mudde, Karin, Haenen, Daniëlle, Al-Lahham, Sa'ad, Roelofsen, Han, Houtman, René, van der Burg, Bart, Mandrup, Susanne, Bonvin, Alexandre M J J, Kalkhoven, Eric, Müller, Michael ORCID: https://orcid.org/0000-0002-5930-9905, Hooiveld, Guido J and Kersten, Sander (2013) Short-chain fatty acids stimulate angiopoietin-like 4 synthesis in human colon adenocarcinoma cells by activating peroxisome proliferator-activated receptor γ. Molecular and Cellular Biology, 33 (7). pp. 1303-1316. ISSN 0270-7306

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Abstract

Angiopoietin-like protein 4 (ANGPTL4/FIAF) has been proposed as a circulating mediator between the gut microbiota and fat storage. Here, we show that transcription and secretion of ANGPTL4 in human T84 and HT29 colon adenocarcinoma cells is highly induced by physiological concentrations of short-chain fatty acids (SCFA). SCFA induce ANGPTL4 by activating the nuclear receptor peroxisome proliferator activated receptor γ (PPARγ), as demonstrated using PPARγ antagonist, PPARγ knockdown, and transactivation assays, which show activation of PPARγ but not PPARα and PPARδ by SCFA. At concentrations required for PPARγ activation and ANGPTL4 induction in colon adenocarcinoma cells, SCFA do not stimulate PPARγ in mouse 3T3-L1 and human SGBS adipocytes, suggesting that SCFA act as selective PPARγ modulators (SPPARM), which is supported by coactivator peptide recruitment assay and structural modeling. Consistent with the notion that fermentation leads to PPAR activation in vivo, feeding mice a diet rich in inulin induced PPAR target genes and pathways in the colon. We conclude that (i) SCFA potently stimulate ANGPTL4 synthesis in human colon adenocarcinoma cells and (ii) SCFA transactivate and bind to PPARγ. Our data point to activation of PPARs as a novel mechanism of gene regulation by SCFA in the colon, in addition to other mechanisms of action of SCFA.

Item Type:	Article
Uncontrolled Keywords:	3t3-l1 cells,adenocarcinoma,adipogenesis,angiopoietins,animals,cell line, tumor,colon,colonic neoplasms,fatty acids, volatile,ht29 cells,humans,inulin,male,mice,mice, inbred c57bl,ppar gamma,transcription, genetic,transcriptional activation
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	10 Jun 2014 20:32
Last Modified:	06 Jun 2024 14:45
URI:	https://ueaeprints.uea.ac.uk/id/eprint/47648
DOI:	10.1128/MCB.00858-12

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