Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery

Saeed, Aram O., Magnusson, Johannes P., Moradi, Emilia, Soliman, Mahmoud, Wang, Wenxin, Stolnik, Snow, Thurecht, Kristofer J., Howdle, Steven M. and Alexander, Cameron (2011) Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery. Bioconjugate Chemistry, 22 (2). pp. 156-168. ISSN 1043-1802

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Abstract

Multifunctional and modular block copolymers prepared from biocompatible monomers and linked by a bioreducible disulfide linkage have been prepared using a combination of ring-opening and atom-transfer radical polymerizations (ATRP). The presence of terminal functionality via ATRP allowed cell-targeting folic acid groups to be attached in a controllable manner, while the block copolymer architecture enabled well-defined nanoparticles to be prepared by a water−oil−water double emulsion procedure to encapsulate DNA with high efficiency. Gene delivery assays in a Calu-3 cell line indicated specific folate-receptor-mediated uptake of the nanoparticles, and triggered release of the DNA payload via cleavage of the disulfide link resulted in enhanced transgene expression compared to nonbioreducible analogues. These materials offer a promising and generic means to deliver a wide variety of therapeutic payloads to cells in a selective and tunable way.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	16 Dec 2013 16:54
Last Modified:	28 Mar 2025 05:45
URI:	https://ueaeprints.uea.ac.uk/id/eprint/46958
DOI:	10.1021/bc100149g

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