Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery

Saeed, Aram O. ORCID: https://orcid.org/0000-0003-2903-5875, Magnusson, Johannes P., Moradi, Emilia, Soliman, Mahmoud, Wang, Wenxin, Stolnik, Snow, Thurecht, Kristofer J., Howdle, Steven M. and Alexander, Cameron (2011) Modular construction of multifunctional bioresponsive cell-targeted nanoparticles for gene delivery. Bioconjugate Chemistry, 22 (2). pp. 156-168. ISSN 1043-1802

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Abstract

Multifunctional and modular block copolymers prepared from biocompatible monomers and linked by a bioreducible disulfide linkage have been prepared using a combination of ring-opening and atom-transfer radical polymerizations (ATRP). The presence of terminal functionality via ATRP allowed cell-targeting folic acid groups to be attached in a controllable manner, while the block copolymer architecture enabled well-defined nanoparticles to be prepared by a water−oil−water double emulsion procedure to encapsulate DNA with high efficiency. Gene delivery assays in a Calu-3 cell line indicated specific folate-receptor-mediated uptake of the nanoparticles, and triggered release of the DNA payload via cleavage of the disulfide link resulted in enhanced transgene expression compared to nonbioreducible analogues. These materials offer a promising and generic means to deliver a wide variety of therapeutic payloads to cells in a selective and tunable way.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	Pure Connector
Date Deposited:	16 Dec 2013 16:54
Last Modified:	25 Sep 2024 11:04
URI:	https://ueaeprints.uea.ac.uk/id/eprint/46958
DOI:	10.1021/bc100149g

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