Outbreak of Pseudomonas aeruginosa infections with PER-1 extended-spectrum beta-lactamase in Warsaw, Poland:further evidence for an international clonal complex

Empel, Joanna, Filczak, Krzysztof, Mrówka, Agnieszka, Hryniewicz, Waleria, Livermore, David M ORCID: https://orcid.org/0000-0002-9856-3703 and Gniadkowski, Marek (2007) Outbreak of Pseudomonas aeruginosa infections with PER-1 extended-spectrum beta-lactamase in Warsaw, Poland:further evidence for an international clonal complex. Journal of Clinical Microbiology, 45 (9). pp. 2829-34. ISSN 0095-1137

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Abstract

Forty-one Pseudomonas aeruginosa isolates with extended-spectrum beta-lactamases (ESBLs) from a hospital in Warsaw, Poland, were analyzed. Thirty-seven isolates from several wards were collected over 9 months in 2003 and 2004. The isolates were recovered from patients with multiple types of infections, mostly respiratory tract and postoperative wound infections. All 41 isolates produced the PER-1 ESBL, originally observed in Turkey but recently also identified in several countries in Europe and the Far East. The bla(PER-1) gene resided within the Tn1213 composite transposon, which was chromosomally located. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) revealed the presence of three separate clones among the isolates. Two of these, corresponding to sequence types (STs) ST244 and ST235, were responsible for parallel outbreaks. Apart from PER-1, all the isolates produced OXA-2 oxacillinase. ST235 isolates additionally expressed a novel enzyme, OXA-74, differing by one amino acid from the OXA-17 ESBL identified originally in PER-1- and OXA-2-positive P. aeruginosa isolates from Ankara, Turkey, in 1992. These earlier Ankara isolates with PER-1, OXA-2, and OXA-17 were also classified into ST235, which is a single-locus variant of two other STs, ST227 and ST230. ST227, ST230, and ST235 all correspond to the recently described clonal complex BG11, which seems to be internationally distributed, having spread in Turkey, Greece, Italy, Hungary, Poland, Sweden, and much of Russia. It is associated with various beta-lactamases, including PER-1 and VIM metalloenzymes. This work further demonstrates the value of MLST of P. aeruginosa.

Item Type:	Article
Uncontrolled Keywords:	bacterial typing techniques,chromosomes, bacterial,cross infection,dna transposable elements,disease outbreaks,electrophoresis, gel, pulsed-field,genes, bacterial,genotype,hospitals,humans,molecular sequence data,poland,pseudomonas infections,pseudomonas aeruginosa,respiratory tract infections,sequence analysis, dna,surgical wound infection,beta-lactamases
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023)
Depositing User:	Pure Connector
Date Deposited:	24 Jan 2014 12:28
Last Modified:	24 Oct 2022 05:46
URI:	https://ueaeprints.uea.ac.uk/id/eprint/46708
DOI:	10.1128/JCM.00997-07

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