Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases

Dhanji, Hiran, Doumith, Michel, Clermont, Olivier, Denamur, Erick, Hope, Russell, Livermore, David M and Woodford, Neil (2010) Real-time PCR for detection of the O25b-ST131 clone of Escherichia coli and its CTX-M-15-like extended-spectrum beta-lactamases. International Journal of Antimicrobial Agents, 36 (4). pp. 355-8. ISSN 1872-7913

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Abstract

CTX-M-15 has become the most prevalent extended-spectrum beta-lactamase amongst Escherichia coli in many countries during the past decade. Its dominance partly reflects the dissemination of an E. coli O25b:H4 ST131 clone that commonly produces this enzyme. We describe rapid real-time polymerase chain reaction (PCR) assays able to detect E. coli belonging to the ST131 clone and to identify bla(CTX-M-15)-like.

Item Type:	Article
Additional Information:	Copyright 2010 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Uncontrolled Keywords:	bacterial typing techniques,dna, bacterial,epidemics,escherichia coli,escherichia coli infections,escherichia coli proteins,humans,molecular epidemiology,polymerase chain reaction,polymorphism, single nucleotide,beta-lactamases
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Epidemiology and Public Health Faculty of Medicine and Health Sciences > Research Groups > Public Health and Health Services Research (former - to 2023)
Depositing User:	Pure Connector
Date Deposited:	20 Jan 2014 16:54
Last Modified:	05 Nov 2025 00:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/46673
DOI:	10.1016/j.ijantimicag.2010.06.007

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