In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression:implications for bacterial chromosome structure

McArthur, Michael and Bibb, Mervyn (2006) In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression:implications for bacterial chromosome structure. Nucleic Acids Research, 34 (19). pp. 5395-401. ISSN 0305-1048

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Abstract

For a bacterium, Streptomyces coelicolor A3(2) contains a relatively large genome (8.7 Mb) with a complex and adaptive pattern of gene regulation. We discovered a correlation between the physical structure of the S.coelicolor genome and the transcriptional activity of the genes therein. Twelve genes were surveyed throughout 72 h of growth for both in vivo sensitivity to DNase I digestion and levels of transcription. DNase I-sensitivity correlated positively with transcript levels, implying that it was predictive of gene expression, and indicating increased accessibility of transcribed DNA. The genome was fractionated based on the sensitivity to DNase I digestion, with the low molecular weight (frequently cut) fraction highly enriched for actively transcribed sequences when compared to the infrequently cut fraction, which was representative of the entire genome. This approach will allow comparison of nucleoid proteins, and any modifications thereof, associated with transcriptionally active and inactive regions of the bacterial genome.

Item Type: Article
Uncontrolled Keywords: chromosomes, fungal,deoxyribonuclease i,genes, fungal,streptomyces coelicolor,transcription, genetic
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science > School of Biological Sciences
Depositing User: Pure Connector
Date Deposited: 20 Jan 2014 16:50
Last Modified: 21 Apr 2020 22:37
URI: https://ueaeprints.uea.ac.uk/id/eprint/46622
DOI: 10.1093/nar/gkl649

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