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ToolsMcArthur, Michael and Bibb, Mervyn (2006) In vivo DNase I sensitivity of the Streptomyces coelicolor chromosome correlates with gene expression:implications for bacterial chromosome structure. Nucleic Acids Research, 34 (19). pp. 5395-401. ISSN 0305-1048
Full text not available from this repository. (Request a copy)Abstract
For a bacterium, Streptomyces coelicolor A3(2) contains a relatively large genome (8.7 Mb) with a complex and adaptive pattern of gene regulation. We discovered a correlation between the physical structure of the S.coelicolor genome and the transcriptional activity of the genes therein. Twelve genes were surveyed throughout 72 h of growth for both in vivo sensitivity to DNase I digestion and levels of transcription. DNase I-sensitivity correlated positively with transcript levels, implying that it was predictive of gene expression, and indicating increased accessibility of transcribed DNA. The genome was fractionated based on the sensitivity to DNase I digestion, with the low molecular weight (frequently cut) fraction highly enriched for actively transcribed sequences when compared to the infrequently cut fraction, which was representative of the entire genome. This approach will allow comparison of nucleoid proteins, and any modifications thereof, associated with transcriptionally active and inactive regions of the bacterial genome.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | chromosomes, fungal,deoxyribonuclease i,genes, fungal,streptomyces coelicolor,transcription, genetic |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Depositing User: | Pure Connector |
| Date Deposited: | 20 Jan 2014 16:50 |
| Last Modified: | 19 Oct 2023 01:14 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/46622 |
| DOI: | 10.1093/nar/gkl649 |
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