NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor

Wilkinson, Adam, Sayer, Heather, Bullard, Desmond, Smith, Andrew, Day, Jonathan, Kieser, Tobias and Bowater, Richard (2003) NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor. Proteins: Structure Function and Bioinformatics, 51 (3). pp. 321-326. ISSN 0887-3585

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Abstract

Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD+ as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD+-dependent DNA ligases.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
Depositing User: EPrints Services
Date Deposited: 01 Oct 2010 13:37
Last Modified: 21 Apr 2020 20:27
URI: https://ueaeprints.uea.ac.uk/id/eprint/463
DOI: 10.1002/prot.10361

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