NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor

Wilkinson, Adam, Sayer, Heather, Bullard, Desmond, Smith, Andrew, Day, Jonathan, Kieser, Tobias and Bowater, Richard ORCID: https://orcid.org/0000-0002-2745-7807 (2003) NAD+-dependent DNA ligases of Mycobacterium tuberculosis and Streptomyces coelicolor. Proteins: Structure Function and Bioinformatics, 51 (3). pp. 321-326. ISSN 0887-3585

Preview

PDF (Wilkinson_Prots_SF&G_2003.pdf) Download (520kB) | Preview

Abstract

Sequencing of the genomes of Mycobacterium tuberculosis H37Rv and Streptomyces coelicolor A3(2) identified putative genes for an NAD+-dependent DNA ligase. We have cloned both open reading frames and overexpressed the protein products in Escherichia coli. In vitro biochemical assays confirm that each of these proteins encodes a functional DNA ligase that uses NAD+ as its cofactor. Expression of either protein is able to complement E. coli GR501, which carries a temperature-sensitive mutation in ligA. Thus, in vitro and in vivo analyses confirm predictions that ligA genes from M. tuberculosis and S. coelicolor are NAD+-dependent DNA ligases.

Item Type:	Article
Uncontrolled Keywords:	sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry
Depositing User:	EPrints Services
Date Deposited:	01 Oct 2010 13:37
Last Modified:	24 Sep 2024 10:08
URI:	https://ueaeprints.uea.ac.uk/id/eprint/463
DOI:	10.1002/prot.10361

Downloads

Actions (login required)

View Item