Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes

Mourtada-Maarabouni, Mirna, Keen, Jennifer, Clark, Jeremy, Cooper, Colin S ORCID: https://orcid.org/0000-0003-2013-8042 and Williams, Gwyn T (2006) Candidate tumor suppressor LUCA-15/RBM5/H37 modulates expression of apoptosis and cell cycle genes. Experimental Cell Research, 312 (10). pp. 1745-52. ISSN 0014-4827

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Abstract

RBM5 (RNA-binding motif protein 5/LUCA-15/H37) is encoded at the lung cancer tumor suppressor locus 3p21.3 and itself has several important characteristics of a tumor suppressor, including both potentiation of apoptosis and inhibition of the cell cycle. Here, we report the effects of both upregulation and downregulation of LUCA-15/RBM5 on gene expression monitored using cDNA microarrays. Many of the genes modulated by LUCA-15/RBM5 are involved in the control of apoptosis, the cell cycle, or both. These effects were confirmed for the most significant genes using real-time RT-PCR and/or Western blotting. In particular, LUCA-15/RBM5 increased the expression of Stat5b and BMP5 and decreased the expression of AIB1 (Amplified In Breast Cancer 1), proto-oncogene Pim-1, caspase antagonist BIRC3 (cIAP-2, MIHC), and CDK2 (cyclin-dependent kinase 2). These effects on multiple genes controlling both apoptosis and proliferation are in line with the functional effects of LUCA-15/RBM5 and indicate that it plays a central role in regulating cell fate consistent with its tumor suppressor activity.

Item Type:	Article
Uncontrolled Keywords:	animals,apoptosis,cell cycle,cell cycle proteins,cell line, tumor,dna-binding proteins,gene expression profiling,gene expression regulation,genes, tumor suppressor,genes, cdc,humans,molecular sequence data,oligonucleotide array sequence analysis,rna-binding proteins,tumor suppressor proteins,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Pure Connector
Date Deposited:	06 Jan 2014 14:12
Last Modified:	06 Jun 2024 14:43
URI:	https://ueaeprints.uea.ac.uk/id/eprint/46155
DOI:	10.1016/j.yexcr.2006.02.009

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