Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism

Sullivan, Matthew J ORCID: https://orcid.org/0000-0003-2276-3132, Gates, Andrew J ORCID: https://orcid.org/0000-0002-4594-5038, Appia-Ayme, Corinne ORCID: https://orcid.org/0000-0002-8315-0789, Rowley, Gary ORCID: https://orcid.org/0000-0002-5421-4333 and Richardson, David J ORCID: https://orcid.org/0000-0002-6847-1832 (2013) Copper control of bacterial nitrous oxide emission and its impact on vitamin B12-dependent metabolism. Proceedings of the National Academy of Sciences of the United States of America (PNAS), 110 (49). pp. 19926-31. ISSN 1091-6490

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Abstract

Global agricultural emissions of the greenhouse gas nitrous oxide (N2O) have increased by around 20% over the last 100 y, but regulation of these emissions and their impact on bacterial cellular metabolism are poorly understood. Denitrifying bacteria convert nitrate in soils to inert di-nitrogen gas (N2) via N2O and the biochemistry of this process has been studied extensively in Paracoccus denitrificans. Here we demonstrate that expression of the gene encoding the nitrous oxide reductase (NosZ), which converts N2O to N2, is regulated in response to the extracellular copper concentration. We show that elevated levels of N2O released as a consequence of decreased cellular NosZ activity lead to the bacterium switching from vitamin B12-dependent to vitamin B12-independent biosynthetic pathways, through the transcriptional modulation of genes controlled by vitamin B12 riboswitches. This inhibitory effect of N2O can be rescued by addition of exogenous vitamin B12.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group
Depositing User:	Pure Connector
Date Deposited:	06 Jan 2014 14:12
Last Modified:	25 Sep 2024 10:57
URI:	https://ueaeprints.uea.ac.uk/id/eprint/45930
DOI:	10.1073/pnas.1314529110

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