Dynamic localization of Tat protein transport machinery components in Streptomyces coelicolor

Willemse, Joost, Ruban-Osmialowska, Beata, Widdick, David, Celler, Katherine, Hutchings, Matthew I., van Wezel, Gilles P. and Palmer, Tracy (2012) Dynamic localization of Tat protein transport machinery components in Streptomyces coelicolor. Journal of Bacteriology, 194 (22). pp. 6272-6281. ISSN 0021-9193

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Abstract

The Tat pathway transports folded proteins across the bacterial cytoplasmic membrane and is a major route of protein export in the Streptomyces genus of bacteria. In this study, we have examined the localization of Tat components in the model organism Streptomyces coelicolor by constructing enhanced green fluorescent protein (eGFP) and mCherry fusions with the TatA, TatB, and TatC proteins. All three components colocalized dynamically in the vegetative hyphae, with foci of each tagged protein being prominent at the tips of emerging germ tubes and of the vegetative hyphae, suggesting that this may be a primary site of Tat secretion. Time-lapse imaging revealed that localization of the Tat components was highly dynamic during tip growth and again demonstrated a strong preference for apical sites in growing hyphae. During aerial hypha formation, TatA-eGFP and TatB-eGFP fusions relocalized to prespore compartments, indicating repositioning of Tat components during the Streptomyces life cycle.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology
Depositing User:	Pure Connector
Date Deposited:	25 Nov 2013 16:50
Last Modified:	14 Dec 2023 01:55
URI:	https://ueaeprints.uea.ac.uk/id/eprint/44952
DOI:	10.1128/JB.01425-12

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