The presence of sodium dodecyl sulphate-stable Abeta dimers is strongly associated with Alzheimer-type dementia

Mc Donald, Jessica M, Savva, George M, Brayne, Carol, Welzel, Alfred T, Forster, Gill, Shankar, Ganesh M, Selkoe, Dennis J, Ince, Paul G and Walsh, Dominic M and Medical Research Council Cognitive Function and Ageing Study (2010) The presence of sodium dodecyl sulphate-stable Abeta dimers is strongly associated with Alzheimer-type dementia. Brain, 133 (5). pp. 1328-1341. ISSN 1460-2156

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Abstract

The molecular pathways leading to Alzheimer-type dementia are not well understood, but the amyloid beta-protein is believed to be centrally involved. The quantity of amyloid beta-protein containing plaques does not correlate well with clinical status, suggesting that if amyloid beta-protein is pathogenic it involves soluble non-plaque material. Using 43 brains from the Newcastle cohort of the population-representative Medical Research Council Cognitive Function and Ageing Study, we examined the relationship between biochemically distinct forms of amyloid beta-protein and the presence of Alzheimer-type dementia. Cortical samples were serially extracted with Tris-buffered saline, Tris-buffered saline containing 1% TX-100 and with 88% formic acid and extracts analysed for amyloid beta-protein by immunoprecipitation/western blotting. The cohort was divisible into those with dementia at death with (n = 14) or without (n = 10) significant Alzheimer-type pathology, and those who were not demented (n = 19). Amyloid beta-protein monomer in extracts produced using Tris-buffered saline and Tris-buffered saline containing 1% TX-100 were strongly associated with Alzheimer type dementia (P

Item Type:	Article
Uncontrolled Keywords:	aged,aged, 80 and over,alzheimer disease,amyloid beta-peptides,blotting, western,cerebral cortex,cohort studies,dementia,drug stability,female,humans,immunoprecipitation,male,protein multimerization,sodium dodecyl sulfate,alzheimer's disease pathology,biochemistry,cognitive impairment
Faculty \ School:
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Health in Later Life (former - to 2017) Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre
Depositing User:	Pure Connector
Date Deposited:	09 Dec 2013 14:38
Last Modified:	04 Dec 2023 01:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/44938
DOI:	10.1093/brain/awq065

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