Freely diffusing versus adsorbed protein: Which better mimics the cellular state of a redox protein?

Doyle, Rose-Marie A.S., Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, Clarke, Thomas A. ORCID: https://orcid.org/0000-0002-6234-1914 and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2013) Freely diffusing versus adsorbed protein: Which better mimics the cellular state of a redox protein? Electrochimica Acta, 110. pp. 73-78. ISSN 0013-4686

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Abstract

Dynamic electrochemistry of adsorbed proteins, often termed protein film electrochemistry (PFE), is widely used for the characterisation of redox proteins. The method provides a powerful alternative to spectroscopic studies that interrogate protein solutions. The reduction potential and electron stoichiometry of redox couples can be defined. The rates of catalytic redox transformations can also be quantified. Often it is considered that the behaviour of the adsorbed protein should match that displayed in solution studies if it is to be relevant to understanding the biological role of the protein. However, the environment of the protein in PFE is fundamentally different from that when it is freely diffusing in solution. As a consequence different behaviours may be expected. This raises the question, which approach is more relevant when aiming to provide insight into the cellular role of the protein? We consider this question here taking as an example the properties of a penta-heme cytochrome NrfB from Escherichia coli. The redox properties of NrfB containing solutions were presented previously (Clarke et al., Journal of Biological Chemistry (2004)). Here we present PFE of NrfB adsorbed on graphite and optically transparent mesoporous, nanocrystalline SnO2 electrodes.

Item Type:	Article
Uncontrolled Keywords:	cytochrome,protein film electrochemistry,potentiometry,nitrite reduction,nrfb
Faculty \ School:	Faculty of Science Faculty of Science > School of Chemistry Faculty of Science > School of Biological Sciences Faculty of Science > School of Natural Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Biophysical Chemistry (former - to 2017) Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory
Depositing User:	Pure Connector
Date Deposited:	11 Nov 2013 16:14
Last Modified:	17 May 2023 00:56
URI:	https://ueaeprints.uea.ac.uk/id/eprint/44388
DOI:	10.1016/j.electacta.2013.03.113

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