High-stringency subtraction for the identification of differentially regulated cDNA clones

Scutt, C.P. and Gilmartin, P.M. (1997) High-stringency subtraction for the identification of differentially regulated cDNA clones. Biotechniques, 23 (3). pp. 468-474. ISSN 0736-6205

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Abstract

The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in ? ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is designed to eliminate the subtraction of differentially expressed cDNAs, which show similarity to constitutive sequences by the incorporation of a novel high-stringency wash step. This method also allows the size-selection of target cDNAs and incorporates an improved procedure for the synthesis of driver DNA used in subtractions.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
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Depositing User: Pure Connector
Date Deposited: 01 Nov 2013 14:22
Last Modified: 21 Apr 2020 22:06
URI: https://ueaeprints.uea.ac.uk/id/eprint/44147
DOI:

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