High-stringency subtraction for the identification of differentially regulated cDNA clones

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Scutt, C.P. and Gilmartin, P.M. (1997) High-stringency subtraction for the identification of differentially regulated cDNA clones. Biotechniques, 23 (3). pp. 468-474. ISSN 0736-6205

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Abstract

The technique of high-stringency subtraction described here facilitates subtractive hybridizations between directional cDNA libraries constructed in ? ZAP® II cloning vectors and represents an improvement on earlier methods for the subtraction of entire cDNA libraries. High-stringency subtraction is designed to eliminate the subtraction of differentially expressed cDNAs, which show similarity to constitutive sequences by the incorporation of a novel high-stringency wash step. This method also allows the size-selection of target cDNAs and incorporates an improved procedure for the synthesis of driver DNA used in subtractions.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	Pure Connector
Date Deposited:	01 Nov 2013 14:22
Last Modified:	24 Oct 2022 04:56
URI:	https://ueaeprints.uea.ac.uk/id/eprint/44147
DOI:

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