The activation of fibroblast growth factors by heparin: Synthesis, structure, and biological activity of heparin-like oligosaccharides

de Paz, José-Luis, Angulo, Jesus ORCID: https://orcid.org/0000-0001-7250-5639, Lassaletta, José-María, Nieto, Pedro M., Redondo-Horcajo, Mariano, Lozano, Rosa M., Giménez-Gallego, Guillermo and Martín-Lomas, Manuel (2001) The activation of fibroblast growth factors by heparin: Synthesis, structure, and biological activity of heparin-like oligosaccharides. ChemBioChem, 2 (9). pp. 673-685. ISSN 1439-4227

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Abstract

An effective strategy has been designed for the synthesis of oligosaccharides of different sizes structurally related to the regular region of heparin; this is illustrated by the preparation of hexasaccharide 1 and octasaccharide 2. This synthetic strategy provides the oligosaccharide sequence containing a D-glucosamine unit at the nonreducing end that is not available either by enzymatic or chemical degradation of heparin. It may permit, after slight modifications, the preparation of oligosaccharide fragments with different charge distribution as well. NMR spectroscopy and molecular dynamics simulations have shown that the overall structure of 1 in solution is a stable right-hand helix with four residues per turn. Hexasaccharide 1 and, most likely, octasaccharide 2 are, therefore, chemically well-defined structural models of naturally occurring heparin-like oligosaccharides for use in binding and biological activity studies. Both compounds 1 and 2 induce the mitogenic activity of acid fibroblast growth factor (FGF1), with the half-maximum activating concentration of 2 being equivalent to that of heparin. Sedimentation equilibrium analysis with compound 2 suggests that heparin-induced FGF1 dimerization is not an absolute requirement for biological activity.

Item Type:	Article
Uncontrolled Keywords:	indicators and reagents,anticoagulants,models, molecular,biotransformation,oligosaccharides,spectrophotometry, ultraviolet,carbohydrate sequence,mitogens,iduronic acid,heparin,fibroblast growth factors,magnetic resonance spectroscopy
Faculty \ School:	Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017)
Depositing User:	Pure Connector
Date Deposited:	21 Oct 2013 19:58
Last Modified:	24 Sep 2024 10:42
URI:	https://ueaeprints.uea.ac.uk/id/eprint/43761
DOI:	10.1002/1439-7633(20010903)2:9%3C673::AID-CBIC673%3E3.0.CO;2-7

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