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ToolsAl-Jamal, Khuloud T., Toma, Francesca M., Yilmazer, Açelya, Ali-Boucetta, Hanene, Nunes, Antonio, Herrero, Maria-Antonia, Tian, Bowen, Eddaoudi, Ayad, Eddaoudi, Ayad, Al-Jamal, Wafa' T., Bianco, Alberto, Prato, Maurizio and Kostarelos, Kostas (2010) Enhanced cellular internalization and gene silencing with a series of cationic dendron-multiwalled carbon nanotube:siRNA complexes. The FASEB Journal, 24 (11). pp. 4354-4365. ISSN 0892-6638
Full text not available from this repository.Abstract
One of the major obstacles to the clinical development of gene silencing by small interfering RNA (siRNA) is its effective cytoplasmic delivery. Carbon nanotubes have been proposed as novel nanomaterials that can offer significant advantages for the intracellular delivery of nucleic acids, such as siRNA. We recently demonstrated in a proof-of-principle study that amino-functionalized multiwalled carbon nanotubes (f-MWNT) can effectively deliver in vivo an siRNA sequence, triggering cell apoptosis that results in human lung xenograft eradication and prolonged survival. In the present study, we demonstrate how a newly synthesized series of polycationic dendron-MWNT constructs with a precisely tailored number of amino functions (dendron generations) can complex and effectively deliver double-stranded siRNA to achieve gene silencing in vitro. A systematic comparison between the f-MWNT series in terms of cellular uptake, cytotoxicity, and siRNA complexation is offered. Significant improvement in siRNA delivery with the dendron-MWNT conjugates is shown, and gene silencing was obtained in 2 human cell lines using 2 different siRNA sequences. The study reveals that through f-MWNT structure-biological function analysis novel nanotube-based siRNA transfer vectors can be designed with minimal cytotoxicity and effective delivery and gene-silencing capabilities.
| Item Type: | Article |
|---|---|
| Additional Information: | An erratum has been issued for this article which corrects the spelling of the 8th author's surname. The corrected spelling is given here. |
| Uncontrolled Keywords: | cations,transfection,models, molecular,hela cells,gene silencing,humans,biological transport,cell line, tumor,rna, small interfering,nanotubes, carbon,cell line,cell survival |
| Faculty \ School: | Faculty of Science > School of Pharmacy (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Drug Delivery and Pharmaceutical Materials (former - to 2017) |
| Depositing User: | Pure Connector |
| Date Deposited: | 05 Oct 2013 00:55 |
| Last Modified: | 22 Nov 2024 01:21 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/43579 |
| DOI: | 10.1096/fj.09-141036 |
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