Mammalian cell entry genes in Streptomyces may provide clues to the evolution of bacterial virulence

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Clark, Laura C., Seipke, Ryan F., Prieto, Pilar, Willemse, Joost, van Wezel, Gilles P., Hutchings, Matthew I. and Hoskisson, Paul A. (2013) Mammalian cell entry genes in Streptomyces may provide clues to the evolution of bacterial virulence. Scientific Reports, 3. ISSN 2045-2322

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Abstract

Understanding the evolution of virulence is key to appreciating the role specific loci play in pathogenicity. Streptomyces species are generally non-pathogenic soil saprophytes, yet within their genome we can find homologues of virulence loci. One example of this is the mammalian cell entry (mce) locus, which has been characterised in Mycobacterium tuberculosis. To investigate the role in Streptomyces we deleted the mce locus and studied its impact on cell survival, morphology and interaction with other soil organisms. Disruption of the mce cluster resulted in virulence towards amoebae (Acanthamoeba polyphaga) and reduced colonization of plant (Arabidopsis) models, indicating these genes may play an important role in Streptomyces survival in the environment. Our data suggest that loss of mce in Streptomyces spp. may have profound effects on survival in a competitive soil environment, and provides insight in to the evolution and selection of these genes as virulence factors in related pathogenic organisms.

Item Type:	Article
Additional Information:	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/
Uncontrolled Keywords:	sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Molecular Microbiology
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	Pure Connector
Date Deposited:	11 Jul 2013 08:49
Last Modified:	21 Oct 2024 23:38
URI:	https://ueaeprints.uea.ac.uk/id/eprint/42976
DOI:	10.1038/srep01109

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