Chan, Simon S. M., McConnell, Ian and Blacklaws, Barbara A. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes. Immunology, 107 (3). pp. 366-372. ISSN 0019-2805
Full text not available from this repository. (Request a copy)Abstract
Dendritic cells (DCs) are professional antigen-presenting cells with a highly immunostimulatory function and the capacity to activate naïve T cells. In recent years the rapid progress in mouse and human DC research can be mainly attributed to the generation of DCs from precursor cells in vitro, although a lack of reagents has hampered DC research in many large animal models. Here we describe the generation and characterization of ovine monocyte-derived DCs in vitro. In addition to the characteristic morphology and non-adherence of DCs, peripheral blood mononuclear cell monocytes cultured with ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) expressed CD11c and major histocompatibility complex (MHC) class II, but did not express CD14. High levels of endocytosis and an ability to stimulate antigen-specific proliferation of CD4 T lymphocytes were also demonstrated.
Item Type: | Article |
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Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
Depositing User: | Users 2731 not found. |
Date Deposited: | 28 Mar 2013 22:16 |
Last Modified: | 01 Aug 2024 12:30 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/42058 |
DOI: | 10.1046/j.1365-2567.2002.01515.x |
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