Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes

Chan, Simon S. M., McConnell, Ian and Blacklaws, Barbara A. (2002) Generation and characterization of ovine dendritic cells derived from peripheral blood monocytes. Immunology, 107 (3). pp. 366-372. ISSN 0019-2805

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Abstract

Dendritic cells (DCs) are professional antigen-presenting cells with a highly immunostimulatory function and the capacity to activate naïve T cells. In recent years the rapid progress in mouse and human DC research can be mainly attributed to the generation of DCs from precursor cells in vitro, although a lack of reagents has hampered DC research in many large animal models. Here we describe the generation and characterization of ovine monocyte-derived DCs in vitro. In addition to the characteristic morphology and non-adherence of DCs, peripheral blood mononuclear cell monocytes cultured with ovine granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) expressed CD11c and major histocompatibility complex (MHC) class II, but did not express CD14. High levels of endocytosis and an ability to stimulate antigen-specific proliferation of CD4 T lymphocytes were also demonstrated.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	Users 2731 not found.
Date Deposited:	28 Mar 2013 22:16
Last Modified:	24 Sep 2024 10:14
URI:	https://ueaeprints.uea.ac.uk/id/eprint/42058
DOI:	10.1046/j.1365-2567.2002.01515.x

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