Glynn, Barry, Lacey, Katrina, O'Grady, Justin, Barry, Thomas, Smith, Terry J. and Maher, Majella (2008) Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA. Journal of Rapid Methods & Automation in Microbiology, 16 (3). pp. 210-221. ISSN 1745-4581
Full text not available from this repository. (Request a copy)Abstract
A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.
Item Type: | Article |
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Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018) |
Depositing User: | Sophie Buckingham |
Date Deposited: | 05 Mar 2013 10:25 |
Last Modified: | 11 Jan 2024 01:22 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/41789 |
DOI: | 10.1111/j.1745-4581.2008.00128.x |
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