Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA

Glynn, Barry, Lacey, Katrina, O'Grady, Justin, Barry, Thomas, Smith, Terry J. and Maher, Majella (2008) Reusable surface plasmon resonance assay for the specific detection of Streptococcus Pneumoniae tmRNA. Journal of Rapid Methods & Automation in Microbiology, 16 (3). pp. 210-221. ISSN 1745-4581

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Abstract

A capture oligonucleotide-based surface plasmon resonance assay was developed for the specific detection of tmRNA from the gram-positive pathogen Streptococcus pneumoniae. Candidate species-specific probes were initially screened on microarrays using labeled in vitro transcribed tmRNA beforebeing subjected to further analysis on a Biacore 2000 biosensor using a variety of unlabeled tmRNA-containing samples. Previous work had demonstrated that there is approximately 1,000 copies/cfu of tmRNA in S. pneumoniae. The natural amplification of this target has the potential to improve the detection capability of a nucleic acid diagnostics assay. In this study, S. pneumoniae-specific probes were designed that enabled the detection of 10 exp12 cRNA copies of the tmRNA target on the Biacore instrument. When evaluated in combination with nucleic acid sequence-based amplification, specific detection of tmRNA from a single S. pneumoniae cell was demonstrated.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Medical Microbiology (former - to 2018)
Depositing User:	Sophie Buckingham
Date Deposited:	05 Mar 2013 10:25
Last Modified:	11 Jan 2024 01:22
URI:	https://ueaeprints.uea.ac.uk/id/eprint/41789
DOI:	10.1111/j.1745-4581.2008.00128.x

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